Species

  • Cynomolgus macaque (Macaca fascicularis)
  • Rhesus macaque (Macaca mulatta)
  • African green monkey (genus Chlorocebus)

Pathogens

Infection method : Intratracheal microspray, intranasal

Objectives

  • Infection tropism
  • Efficacy assessment of vaccines or therapeutics
  • Evaluation of systemic or topical (incl nebulization) administrations

Readouts

  • Clinical follow-up
  • Core body temperature : continuous monitoring using an implantable data logger
  • Clinical pathology : biochemistry and complete blood count with differential) in a BSL1/2/3 setting thanks to state-of-the-art biological analyzers
  • Bronchoalveolar lavage
  • Nasal & nasopharyngeal swabs
  • Immunomonitoring
  • Viral detection and quantification by RT-qPCR
  • Viral titration using  using the Median Tissue Culture Infectious Dose (TCID50) assay
  • Assessment of the host response by seroneutralization assays
  • Histopathology
  • Custom readouts

Scientific publications

  • African Green Monkey model of SARS-CoV-2 infection shows mild lung pathology and viral replication in the airways.
    Confais J, Toussenot M, Julien T, Ployart S, Pizzorno A, Rosa-Calatrava M, Contamin H.
    Immunotherapies & Innovations for Infectious Diseases congress, 9-10th December, 2020.
  • A respiratory syncytial virus (RSV) F protein nanoparticle vaccine focuses antibody responses to a conserved neutralization domain.
    Swanson K. A et al. (cf Material & methods)
    Science Immunology, 2020 May 01; 5 (47).
    Sci Immunol
  • Development of a cynomolgus macaque model of infection with hRSV to evaluate therapeutic approaches.
    Blanc SC et al.
    Infectious Diseases Models for Innovative Therapies (IDMIT) Symposium 2016, 10 June 2016, Paris.
    IDMIT
  • Development of a cynomolgus macaque model of infection with hRSV to evaluate therapeutic approaches.
    Blanc SC et al.
    Therapeutic Monoclonal Antibodies for Respiratoy Diseases (TMARD): current challenges and perspectives, 31 March-1 April 2016, Tours.
    TMARD
  • Respiratory syncytial virus infection in macaques is not suppressed by intranasal sprays of pyrimidine biosynthesis inhibitors.
    Grandin C et al.
    Antiviral Res. 2016 Jan 19;125:58-62.
    ScienceDirect