Respiratory viral infections: hMPV, hRSV, measles, SARS-CoV-2

Infection method : Intratracheal microspray, intranasal

Clinical follow-up
Core body temperature : continuous monitoring using an implantable data logger
Clinical pathology : biochemistry and complete blood count with differential) in a BSL1/2/3 setting thanks to state-of-the-art biological analyzers
Bronchoalveolar lavage
Nasal & nasopharyngeal swabs
Immunomonitoring
Viral detection and quantification by RT-qPCR
Viral titration using using the Median Tissue Culture Infectious Dose (TCID50) assay
Assessment of the host response by seroneutralization assays
Histopathology
Custom readouts

African Green Monkey model of SARS-CoV-2 infection shows mild lung pathology and viral replication in the airways.
Confais J, Toussenot M, Julien T, Ployart S, Pizzorno A, Rosa-Calatrava M, Contamin H.
Immunotherapies & Innovations for Infectious Diseases congress, 9-10^th December, 2020.
A respiratory syncytial virus (RSV) F protein nanoparticle vaccine focuses antibody responses to a conserved neutralization domain.
Swanson K. A et al. (cf Material & methods)
Science Immunology, 2020 May 01; 5 (47).
Sci Immunol
Development of a cynomolgus macaque model of infection with hRSV to evaluate therapeutic approaches.
Blanc SC et al.
Infectious Diseases Models for Innovative Therapies (IDMIT) Symposium 2016, 10 June 2016, Paris.
IDMIT
Development of a cynomolgus macaque model of infection with hRSV to evaluate therapeutic approaches.
Blanc SC et al.
Therapeutic Monoclonal Antibodies for Respiratoy Diseases (TMARD): current challenges and perspectives, 31 March-1 April 2016, Tours.
TMARD
Respiratory syncytial virus infection in macaques is not suppressed by intranasal sprays of pyrimidine biosynthesis inhibitors.
Grandin C et al.
Antiviral Res. 2016 Jan 19;125:58-62.
ScienceDirect