Species
- Cynomolgus macaque (Macaca fascicularis)
- Rhesus macaque (Macaca mulatta)
- African green monkey (genus Chlorocebus)
Pathogens
- Human Respiratory Syncytial Virus (hRSV)
- Human Metapneumovirus (hMPV)
- SARS-CoV-2 / COVID-19
- Measles
Infection method : Intratracheal microspray, intranasal
Objectives
- Infection tropism
- Efficacy assessment of vaccines or therapeutics
- Evaluation of systemic or topical (incl nebulization) administrations
Readouts
- Clinical follow-up
- Core body temperature : continuous monitoring using an implantable data logger
- Clinical pathology : biochemistry and complete blood count with differential) in a BSL1/2/3 setting thanks to state-of-the-art biological analyzers
- Bronchoalveolar lavage
- Nasal & nasopharyngeal swabs
- Immunomonitoring
- Viral detection and quantification by RT-qPCR
- Viral titration using using the Median Tissue Culture Infectious Dose (TCID50) assay
- Assessment of the host response by seroneutralization assays
- Histopathology
- Custom readouts
Scientific publications
- African Green Monkey model of SARS-CoV-2 infection shows mild lung pathology and viral replication in the airways.
Confais J, Toussenot M, Julien T, Ployart S, Pizzorno A, Rosa-Calatrava M, Contamin H.
Immunotherapies & Innovations for Infectious Diseases congress, 9-10th December, 2020. - A respiratory syncytial virus (RSV) F protein nanoparticle vaccine focuses antibody responses to a conserved neutralization domain.
Swanson K. A et al. (cf Material & methods)
Science Immunology, 2020 May 01; 5 (47).
Sci Immunol - Development of a cynomolgus macaque model of infection with hRSV to evaluate therapeutic approaches.
Blanc SC et al.
Infectious Diseases Models for Innovative Therapies (IDMIT) Symposium 2016, 10 June 2016, Paris.
IDMIT - Development of a cynomolgus macaque model of infection with hRSV to evaluate therapeutic approaches.
Blanc SC et al.
Therapeutic Monoclonal Antibodies for Respiratoy Diseases (TMARD): current challenges and perspectives, 31 March-1 April 2016, Tours.
TMARD - Respiratory syncytial virus infection in macaques is not suppressed by intranasal sprays of pyrimidine biosynthesis inhibitors.
Grandin C et al.
Antiviral Res. 2016 Jan 19;125:58-62.
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